nACh subunit a4

Click on sections of picture to learn more about research on individual amino acids. Numbering of the nACh a4 subunit sequences starts at the end of the signal sequence. Only amino acids in the mature protein are shown.


amino acid all M3 M2 M1-M2 Linker M1 M2-M3 Linker N-Terminus M3-M4 Linker M4

nACh a4 -- GENBANK

nACh a4 -- FASTA

nACh a4 -- Alignment

 

 

 

 

 

 

 

 

 


N-Terminus

Residues with mutations: [ Glu 176 ] Arg 1 Ala 2 His 3 Ala 4 Glu 5 Glu 6 Arg 7 Leu 8 Leu 9 Lys 10 Lys 11 Leu 12 Phe 13 Ser 14 Gly 15 Tyr 16 Asn 17 Lys 18 Trp 19 Ser 20 Arg 21 Pro 22 Val 23 Ala 24 Asn 25 Ile 26 Ser 27 Asp 28 Val 29 Val 30 Leu 31 Val 32 Arg 33 Phe 34 Gly 35 Leu 36 Ser 37 Ile 38 Ala 39 Gln 40 Leu 41 Ile 42 Asp 43 Val 44 Asp 45 Glu 46 Lys 47 Asn 48 Gln 49 Met 50 Met 51 Thr 52 Thr 53 Asn 54 Val 55 Trp 56 Val 57 Lys 58 Gln 59 Glu 60 Trp 61 His 62 Asp 63 Tyr 64 Lys 65 Leu 66 Arg 67 Trp 68 Asp 69 Pro 70 Ala 71 Asp 72 Tyr 73 Glu 74 Asn 75 Val 76 Thr 77 Ser 78 Ile 79 Arg 80 Ile 81 Pro 82 Ser 83 Glu 84 Leu 85 Ile 86 Trp 87 Arg 88 Pro 89 Asp 90 Ile 91 Val 92 Leu 93 Tyr 94 Asn 95 Asn 96 Ala 97 Asp 98 Gly 99 Asp 100 Phe 101 Ala 102 Val 103 Thr 104 His 105 Leu 106 Thr 107 Lys 108 Ala 109 His 110 Leu 111 Phe 112 His 113 Asp 114 Gly 115 Arg 116 Val 117 Gln 118 Trp 119 Thr 120 Pro 121 Pro 122 Ala 123 Ile 124 Tyr 125 Lys 126 Ser 127 Ser 128 Cys 129 Ser 130 Ile 131 Asp 132 Val 133 Thr 134 Phe 135 Phe 136 Pro 137 Phe 138 Asp 139 Gln 140 Gln 141 Asn 142 Cys 143 Thr 144 Met 145 Lys 146 Phe 147 Gly 148 Ser 149 Trp 150 Thr 151 Tyr 152 Asp 153 Lys 154 Ala 155 Lys 156 Ile 157 Asp 158 Leu 159 Val 160 Asn 161 Met 162 His 163 Ser 164 Arg 165 Val 166 Asp 167 Gln 168 Leu 169 Asp 170 Phe 171 Trp 172 Glu 173 Ser 174 Gly 175 Glu 176 Trp 177 Val 178 Ile 179 Val 180 Asp 181 Ala 182 Val 183 Gly 184 Thr 185 Tyr 186 Asn 187 Thr 188 Arg 189 Lys 190 Tyr 191 Glu 192 Cys 193 Cys 194 Ala 195 Glu 196 Ile 197 Tyr 198 Pro 199 Asp 200 Ile 201 Thr 202 Tyr 203 Ala 204 Phe 205 Val 206 Ile 207 Arg 209 Arg 208 Leu 210 Pro 211

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M1

Tyr 234 Phe 233 Val 232 Leu 231 Thr 229 Val 230 Leu 228 Cys 227 Ser 226 Ile 225 Leu 224 Leu 223 Cys 222 Pro 221 Ile 220 Ile 219 Leu 218 Asn 217 Ile 216 Thr 215 Tyr 214 Phe 213 Leu 212

Residues with mutations: [ Cys 227 ]

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M1-M2 Linker

Leu 235 Pro 236 Ser 237 Glu 238 Cys 239 Gly 240 Glu 241 Lys 242

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M2


Residues with mutations: [ Ser 248 ] [ Ser 252 ] [ +Leu 260 ] Ile 243 Thr 244 Leu 245 Cys 246 Ile 247 Ser 248 Val 249 Leu 250 Leu 251 Ser 252 Leu 253 Thr 254 Val 255 Phe 256 Leu 257 Leu 258 Leu 259 Ile 260 Thr 261 +L260

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M2-M3 Linker

GLu 262 Ile 263 Ile 264 Pro 265 Ser 266 Thr 26 Ser 268 Leu 269 Val 270 Ile 271

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M3

Pro 272 Leu 273 Ile 274 Gly 275 Glu 276 Tyr 277 Leu 278 Leu 279 Phe 280 Thr 281 Met 282 Ile 283 Phe 284 Val 285 Thr 286 Leu 287 Ser 288 Ile 289 Val 290

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M3-M4 Linker


Residues with mutations: [ Glu 549 ] [ Phe 553 ] [ Glu 557 ] Ile 291 Thr 292 Val 293 Phe 294 Val 295 Leu 296 Asn 297 Val 298 His 299 His 300 Arg 301 Ser 302 Pro 303 Arg 304 Thr 305 His 306 Thr 307 Met 308 Pro 309 Thr 310 Trp 311 Val 312 Arg 313 Arg 314 Val 315 Phe 316 Leu 317 Asp 318 Ile 319 Val 320 Pro 321 Arg 322 Leu 323 Leu 324 Leu 325 Met 326 Lys 327 Arg 328 Pro 329 Ser 330 Val 331 Val 332 Lys 333 Asp 334 Asn 335 Cys 336 Arg 337 Arg 338 Leu 339 Ile 340 Glu 341 Ser 342 Met 343 His 344 Lys 345 Met 346 Ala 347 Ser 348 Ala 349 Pro 350 Arg 351 Phe 352 Trp 353 Pro 354 Glu 355 Pro 356 Glu 357 Gly 358 Glu 359 Pro 360 Pro 361 Ala 362 Thr 363 Ser 364 Gly 365 Thr 366 Gln 367 Ser 368 Leu 369 His 370 Pro 371 Pro 372 Ser 373 Pro 374 Ser 375 Phe 376 Cts 377 Val 378 Pro 379 Leu 380 Asp 381 Val 382 Pro 383 Ala 384 Glu 385 Pro 386 Gly 387 Pro 388 Ser 389 Cys 390 Lys 391 Ser 392 Pro 393 Ser 394 Asp 395 Gln 396 Leu 397 Pro 398 Pro 399 Gln 400 Gln 401 Pro 402 Leu 403 Glu 404 Ala 405 Glu 406 Lys 407 Ala 408 Ser 409 Pro 410 His 411 Pro 412 Ser 413 Pro 414 Gly 415 Pro 416 Cys 417 Arg 418 Pro 419 Pro 420 His 421 Gly 422 Thr 423 Gln 424 Ala 425 Pro 426 Gly 427 Leu 428 Ala 429 Lys 430 Ala 431 Arg 432 Ser 433 Leu 434 Ser 435 Val 436 Gln 437 His 438 Met 439 Ser 440 Ser 441 Pro 442 Gly 443 Glu 444 Ala 445 Val 446 Glu 447 Gly 448 Gly 449 Val 450 Arg 451 Cys 452 Arg 453 Ser 454 Arg 455 Ser 456 Ile 457 Gln 458 Tyr 459 Cys 460 Val 461 Pro 462 Arg 463 Asp 464 Asp 465 Ala 466 Ala 467 Pro 468 Glu 469 Ala 470 Asp 471 Gly 472 Gln 473 Ala 474 Ala 475 Gly 476 Ala 477 Leu 478 Ala 479 Ser 480 Arg 481 Asn 482 Thr 483 His 484 Ser 485 Ala 486 Glu 487 Leu 488 Pro 489 Pro 490 Pro 491 Asp 492 Gln 493 Pro 494 Ser 495 Pro 496 Cys 497 Lys 498 Cys 499 Thr 500 Cys 501 Lys 502 Lys 503 Glu 504 Pro 505 Ser 506 Ser 507 Val 508 Ser 509 Pro 510 Ser 511 Ala 512 Thr 513 Val 514 Lys 515 Thr 516 Arg 517 Ser 518 Thr 519 Lys 520 Ala 521 Pro 522 Pro 523 Pro 524 His 525 Leu 526 Pro 527 Leu 528 Ser 529 Pro 530 Ala 531 Leu 532 Thr 533 Arg 534 Ala 535 Val 536 Glu 537 Gly 538 Val 539 Gln 540 Tyr 541 Ile 542 Ala 543 Asp 544 His 545 Leu 546 Lys 547 Ala 548 Glu 549 Asp 550 Thr 551 Asp 552 Phe 553 Ser 554 Val 555 Lys 556 Glu 557 Asp 558 Trp 559 Lys 560 Tyr 561 Val 562 Ala 563 Met 564 Val 565 Ile 566 Asp 567 Arg 568 Ile 569 Phe 570 Leu 571 Trp 572

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M4

Met 573 Phe 574 Ile 575 Ile 576 Val 577 Cys 578 Leu 579 Leu 580 Gly 581 Thr 582 Val 583 Gly 584 Leu 585 Phe 586 Leu 587 Pro 588 Pro 589 Trp 590 Leu 591 Ala 592 Gly 593 Met 594 Ile 595

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Alignment

N-Terminus First Membrane Spanning Second Membrane Spanning Third Membrane Spanning Outside the Cell Inside the Cell C-Terminus