Human nACh a4 -- Phe 553

Species Original Mutated to Mutation
Human Phe 553    
Rat Equivalent Phe 588 Arg

F588R

Mouse Equivalent Phe 587    

rPhe588Arg

This mutation had little effect on the single-channel conductance. When it was combined with R436D, the double mutation produced conductance that was greater than the sum of the individual mutations. When it was combined with R440A, there was no such synergy. In a triple mutation of R432Q, R436D, and R440A, the single-channel conductance increased substantially.

Hales TG, Dunlop JI, Deeb TZ, Carland JE, Kelley SP, Lambert JJ, Peters JA (2006) Common determinants of single channel conductance within the large cytoplasmic loop of 5-HT3 and alpha 4beta 2 nicotinic acetylcholine receptors. The Journal of Biological Chemistry [Epub ahead of print]

 

See Equivalent Mutation in R436D

 

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