Human 5-HT3A -- Trp 97

Species Original Mutated to Mutation
Human Trp 97  

 

Rat Equivalent Trp 96    
Mouse Equivalent Trp 102

Tyr

Ser

W102Y

W102S

 

mTrp102Tyr

No specific binding of either [3H]granisetron or [3H]mCPBG was observed with this mutant. When transfected into HEK293 cells, the mutant had no electrophysiological response even to high concentrations of 5-HT.

 

Spier AD, Lumis SCR (2000) The Role of Tryptophan Residues in the 5-Hydroxytryptamine3 Receptor Ligand Binding Domain. The Journal of Biological Chemistry 275(8): 5620-5625

 

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mTrp102Ser

No specific binding to either [3H] Granisetron or [3H] mCPBG was shown with this mutant. When transfected into HEK293 cells, no electrophysiological responses were shown to </= 1mM 5-HT.

 

Spier AD, Lumis SCR (2000) The Role of Tryptophan Residues in the 5-Hydroxytryptamine3 Receptor Ligand Binding Domain. The Journal of Biological Chemistry 275(8): 5620-5625

 

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