Human 5-HT3A -- Ser 379

Species Original Mutated to Mutation
Human Ser 379  


Rat Equivalent Ser 384    
Mouse Equivalent Ser 384 Ala S384A


Simultaneous mutation of this residue and all other putative phosphorylation sites (replacement by alanine) in the mouse (cloned from NCB-20 cells) 5-HT3A receptor's intracellular loops (TM1-2 and TM3-4) failed to affect modulation by ethanol. Recombinant 5-HT3A receptors were expressed in Xenopus Oocytes (cRNA injection) and 5-HT-evoked currents were recorded using two-electrode voltage-clamp. The effect of replacing intracellular Ser, Thr and Tyr (by Ala) was to shift the 5-HT concentration-response relationship slightly to the left (more potent).

Machu TK, Coultrap SJ, Waugh MD, Hamilton ME. (1999) Mutation of putative phosphorylation sites in the 5-hydroxytryptamine3 receptor does not eliminate its modulation by ethanol. Alcohol Clin Exp Res. 23(1):12-7

back to top
go back to 5-HT3A

Previous Next