Human 5-HT3A -- Ser 285

Species Original Mutated to Mutation
Human Ser 285  

 

Rat Equivalent Ser 284    
Mouse Equivalent Ser 290 Cys S290C

 

mSer290Cys

No current was detected after the application of 5-HT up to 1mM or when it was perfused with 10mM dithiothreitol for 3 minutes. After the application of 1mM MTSET with 10uM 5-HT, no current was detected. After the application of 1mM MTSES with 10uM 5-HT, no current was detected.

 

Reeves DC, Goren EN, Akabas MH, Lummis SCR (2001) Structural and Electrostatic Properties of the 5-HT3 Receptor Pore Revealed by Substituted Cysteine Accessibility Mutagenesis. The Journal of Biological Chemistry 276(45):42035-42042

 

  Wild Type S290C
EC50 1.67 +/- 0.09 0.92 +/- 0.11
Hill Coefficient 2.0 +/- 0.1 2.3 +/- 0.3
Mg Inhibition (before MTSET) -41 +/- 3 -15 +/- 2
Mg Inhibition (after MTSET) -39 +/- 3 -28 +/- 3
Deactivation T50 ratio 0.95 +/- 0.03 0.91 +/- 0.06

 

Panicker S, Cruz H, Arrabit C, Slesinger PA (2002) Evidence for a Centrally Located Gate in the Pore of a Serotonin-Gated Ion Channel. The Journal of Neuroscience 22(5): 1629-1639

 

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