Human 5-HT3A -- Ser 248

Species Original Mutated to Mutation
Human Ser 248 Thr

S248T

Rat Equivalent Ser 247    
Mouse Equivalent Ser 253    

hSer248Thr

The kinetics of desensitization of the human and guinea pig 5-HT3A receptors were studied using the patch-clamp technique. The human 5-HT3A receptor desensitized more quickly than did the guinea pig 5-HT3A receptor. The cause of the different desensitization kinetics was determined by constructing chimeras of human and guinea pig 5-HT3A subunits. When the human 5-HT3A residue Ser248 was mutated to Thr, which is found in guinea pig, this resulted in slower desensitization of the human receptor.

 

Lobitz N,Gisselmann G,Hatt H and Wetzel CH (2001) A Single Amino-Acid in the TM1 Domin Is an Important Determinant of the Desensitization Kinetics of Recombinant Human and Guinea Pig alpha-Homomeric 5-Hydroxytryptamine Type 3 Receptors. Molecular Pharmacology 59:844-851

 

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