Human 5-HT3A -- Pro 296

Species Original Mutated to Mutation
Human Pro 296  

 

Rat Equivalent Pro 295    
Mouse Equivalent Pro 301 Ala P301A

 

mPro301Ala

Specific Binding of [3H]granisetron revealed Kd values of 0.20+/- 0.07nM. Using pAb120, the mutant receptor was shown to be localized on the cell surface, however they were non-functional when using whole cell patch clamp.

Deane CM, Lummis SCR (2001) The Role and Predicted Propensity of Conserved Proline Residues in the 5-HT3 Receptor. The Journal of Biological Chemistry 276(41):37962-37966

 

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