Human 5-HT3A -- Pro 165

Species Original Mutated to Mutation
Human Pro 165  

 

Rat Equivalent Pro 164    
Mouse Equivalent Pro 171 Ala P171A

 

mPro171Ala

Mutation resulted in the ablation of specific binding. Labeling was observed in permeabilized preparations using an antibody specific for the N terminus of the 5-HT3A subunit.

Because of the cis nature of this proline, its adjacent residues were also mutated: mPhe169 and mPhe171 to alternative "favorable" and "non-favorable residues."

Deane CM, Lummis SCR (2001) The Role and Predicted Propensity of Conserved Proline Residues in the 5-HT3 Receptor. The Journal of Biological Chemistry 276(41):37962-37966

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